Sensitivity to fungicides of Phytophthora infestans (Mont.) de Bary in Chapingo, Mexico
Chemical control of plant diseases exerts high selection pressure for the development of pathogen resistance to fungicides. The objectives of this study were to quantify the existence of late blight in Solanum tuberosum L., detect the presence of variants of the oomycete resistant to agrochemicals and compare the different levels of resistance in the isolates from one cycle to another. Plots of the potato cv. Fianna, susceptible to late blight (Phytophthora infestans [Mont] de Bary), were exposed to natural infection of the pathogen to detect the presence of variants of the oomycete resistant to the agrochemicals from one cycle to another, and from in vivo with respect to in vitro conditions, by applying a half dose of fungicides. The disease reached 100 % final foliar infection in treatments with ciazofamid in 2011 and with fosetyl-Al in 2012. On the other hand, mandipropamid allowed only 21-22 % infection in both years in the field. However, 49 % of the P. infestans population was susceptible, 39 % was intermediate, and 19 % was resistant to this product in vitro. Plots treated with fosetyl-Al reached 80-100 % infection in the field, while in vitro 88 and 46 % of the P. infestans populations showed intermediate resistance when exposed to high dosages of the product, and 62 % were resistant at low dosage. Isolates from field plots with natural infection exposed to ciazofamid were all sensitive in vitro. The inconsistencies of the oomycete regarding fungicide resistance/sensitivity under in vivo/in vitro conditions, as well from one year to another, do not support behavior predictability. These results expose the great genetic plasticity of the oomycete in relation to its sensitivity to fungicides.
El control químico de las enfermedades de las plantas ejerce gran presión de selección en el desarrollo de resistencia del patógeno a fungicidas. Los objetivos del presente estudio fueron cuantificar la existencia de tizón tardío en Solanum tuberosum L., detectar la presencia de variantes del oomiceto resistentes a agroquímicos y comparar los diferentes niveles de resistencia en los aislados de un ciclo a otro. Se expusieron parcelas de papa var. Fianna, susceptibles al tizón tardío (Phytophthora infestans [Mont.] de Bary), a la infección natural del patógeno para detectar la presencia de variantes del oomiceto resistentes a agroquímicos de un ciclo a otro, y bajo condiciones in vivo respecto a in vitro mediante la aplicación de medias dosis de fungicidas. La enfermedad alcanzó 100 % de infección foliar final en tratamientos con ciazofamida en 2011 y con fosetil-Al en 2012. Por otro lado, la mandipropamida permitió solamente de 21 a 22 % de infección en ambos años en campo. No obstante, 49 % de la población de P. infestans resultó susceptible, 39 % intermedia y 19 % fue resistente a este producto in vitro. Las parcelas tratadas con fosetil-Al alcanzaron de 80 a 100 % de infección en campo y 88 % in vitro. El 46 % de las poblaciones de P. infestans mostraron resistencia intermedia cuando se expusieron a dosis altas del producto y 62 % fueron resistentes a dosis bajas. Los aislados de parcelas con infección natural expuestos a ciazofamida fueron sensibles in vitro. Las inconsistencias del oomiceto en relación a la resistencia/susceptibilidad a los fungicidas bajo condiciones in vitro/in vivo, así como de un año a otro, no explican la predictibilidad del comportamiento. Estos resultados exponen la gran plasticidad genética del oomiceto en relación con su sensibilidad a los fungicidas.
Bibliographic data
| Translated title: | Sensibilidad a fungicidas del Phytophthora infestans (Mont.) de Bary en Chapingo, México |
|---|---|
| Journal Title: | Revista Chapingo. Serie Horticultura |
| First author: | Héctor Lozoya-Saldaña |
| Other Authors: | Martha Nayeli Martha Nayeli Robledo-Esqueda; Patricia Rivas-Valencia; Sergio Sandoval-Islas; María Teresa Beryl Colinas y León; Cristian Nava-Díaz |
| Palabras clave: | |
| Traslated Keywords: | |
| Language: | English Spanish |
| Get full text: | https://chapingo.mx/revistas/horticultura/contenido.php?id_articulo=2436&id_revistas=1&id_revista_numero=248 |
| Resource type: | Journal Article |
| Source: | Revista Chapingo. Serie Horticultura; Vol 23, No 3 (Year 2017). |
| DOI: | http://dx.doi.org/10.5154/r.rchsh.2017.01.004 |
| Publisher: | Universidad Autónoma Chapingo |
| Usage rights: | Reconocimiento - NoComercial (by-nc) |
| Knowledge areas / Categories: | Life Sciences --> Agriculture, Multidisciplinary Life Sciences --> Horticulture |
Statistical data
- Views
- Consultations
- Citation style
- Share
- Export record
- Favourites
Bibliometric data
WOS
| Bibliography: | Abad, J. A., & Ochoa, C. (1995). Historical evidence on occurrence of the late blight of potato, tomato and pear melon in the Andes of South America. In: Dowlwey, L. J., Bannon, E., Cooke, R. L., Keane, T., & Sullivan, E. (Eds). Phytophthora infestans 150 (pp: 36-41). Dublin, Ireland: Boole Press Ltd. Adler, N. F., Erselius, L. J., Chacón, M. G., Flier, W. G., Ordóñez, M. E., Kroon, L., & Forbes, G. A. (2004). Genetic diversity of Phytophthora infestans sensu lato in Ecuador provides new insight into the origin of this important plant pathogen. Phytopathology, 94(2), 154-162. doi: 10.1094/PHYTO.2004.94.2.154 Alarcón-Rodríguez, N. M., Lozoya-Saldaña, H., Valadez-Moctezuma, E., García-Mateos, R., & Colinas-León, M. T. (2013). Genetic diversity of potato late blight, Phytophthora infestans (Mont) de Bary, at Chapingo, México. Agrociencia, 47(6) 593-607. Retrieved from http://www.scielo.org.mx/scielo.php?pid=S1405-31952013000600006&script=sci_abstract&tlng=en Blum, M., Boehler, M., Randall, E., Young, V., Csukai, M., & Kraus, S. (2010). Mandipropamid targets the cellulose synthase like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans. Molecular Plant Pathology, 11(2), 227- 243. doi: 10.1111/j.1364-3703.2009.00604.x Brent, K. J., & Hollomon, D. W. (2007). Fungicide resistance in crop pathogens: How can it be managed?. Belgium: Fungicide Resistance Action Committee. Retrieved from http://www.frac.info/docs/default-source/publications/monographs/monograph-1.pdf Campbell, C. L., & Madden, L. V. (1990). Introduction to plant disease epidemiology. New York, USA: Wiley. Cohen, Y., Rubin, E., Hadad, T., Gotlieb, D., Sierotzki, H., & Gisi, U. (2007). Sensitivity of Phytophthora infestans to mandipropamid and the effect of enforced selection pressure in the field. Plant Pathology, 56(5), 836-842. doi: 10.1111/j.1365-3059.2007.01625.x Damicone, J. (2004). Fungicide resistance management. Oklahoma: Cooperative Oklahoma Extension Service. Retrieved from http://pods.dasnr.okstate.edu/docushare/dsweb/Get/Rendition-3508/F-7663web.pdf Davidse, L., Looijen, C. D., Turkesnteen, L. J., & van der Wal, D. (1981). Occurrence of metalaxyl resistant strains of Phytophthora infestans in Dutch potato fields. Netherlands Journal of Plant Pathology, 87(2), 65-68. doi: 10.1007/BF01976658 Deahl, K. L., DeMuth, S. P., & Fry, W. E. (1995a). Genetic and phenotypic diversity in populations of Phytophthora infestans in the United States of America. In: Dowlwey, L. J., Bannon, E., Cooke, R. L., Keane, T., & Sullivan, E. (Eds), Phytophthora infestans 150 (pp: 362). Dublin, Ireland: Boole Press Ltd . Deahl, K. L, DeMuth, S. P., Sinden, S. L., & Rivera-Peña, A. (1995b). Identification of mating types and metalaxyl resistance in North American populations of Phytophthora infestans. American Potato Journal, 72(1), 35-49. Díaz-de la Cruz, J. B., Lozoya-Saldaña, H., Sahagun-Castellanos, J., & Peña-Lomelí, A. (2014). The pathosystem Solanum tuberosum L.-Phytophthora infestans (Mont.) de Bary in Chapingo. México. Expected, observed, and simulated. American Journal of Potato Research, 91(3), 312-326. doi: 10.1007/s12230-013-9351-y Doster, M. A., Milgroom, M. G., & Fry, W. E. (1990). Quantification of factors influencing potato late blight suppression and selection for metalaxyl resistance in Phytophthora infestans: A simulation approach. Phytopathology, 80, 1190-1198. doi: 10.1094/Phyto-80-1190 Dowley, L. J., & O’Sullivan, E. (1985). Monitoring metalaxyl resistance in population of Phytophthora infestans. Potato Research, 28, 531-534. Drenth, A., Goodwin, S. B., Fry, W. E., & Davidse, L. C. (1993). Genotypic diversity of Phytophthora infestans in the Netherlands revealed by DNA polymorphism. Phytopathology, 83(10), 1087-1092. doi: 10.1094/Phyto-83-1087 Erwin, D., & Ribeiro, O. (1996). Introduction to the genus Phytophthora. In: Edwin D. and Ribeiro,O. (Eds.). Phytophthora diseases worldwide (pp. 1-7). St. Paul, Minnesota: American Phytopathological Society. Evenhuis, A., Schepers, H. T., Bus, C. B., & Stegeman, W. (1996). Synergy of cymoxanil and mancozeb when used to control potato late blight. Potato Research, 39(4), 551-559. doi: 10.1007/BF02358474 Forbes, G. A., Escobar, X. C., Ayala, C. C., Revelo, J., Ordoñez, M. E., Fry, B. A., Dovccett, K., & Fry, W. E. (1997). Population genetic structure of Phytophthora infestans in Ecuador. Phytopathology, 87(4), 375-380. doi: 10.1094/PHYTO.1997.87.4.375 Garay-Serrano, E., Fernández-Pavía, S. P., Rodríguez-Alvarado, G., Flier, W. G., Lozoya-Saldaña, H., Rojas-Martínez, R. I., Goss, E. M., & Grünwald, N. J. (2007). First report of haplotype I-b of Phytophthora infestans (Mont) de Bary in Central Mexico. Plant disease, 91(7), 909. doi: 10.1094/PDIS-91-7-0909B Gisi, U., & Cohen, Y. (1996). Resistance to phenylamide fungicides: A case study with Phytophthora infestans involving mating type and race structure. Annual Review of Phytopathology, 34, 549-572. doi: 10.1146/annurev.phyto.34.1.549 Gómez, D. E., & Reis, E. M. (2011). Inductores abióticos de resistencia contra fitopatógenos. Química viva, 10, 6-17. Goodwin, S. B. (1996) Origin and ecology of Phytophthora infestans. Revista Mexicana de Fitopatologia , 14, 143-147. Goodwin, S. B., Sujkowski, L. S., & Fry, W. E. (1995). Rapid evolution of pathogenicity with clonal lineages of the potato late blight disease fungus. Phytopathology, 85(6), 669-76. Griffiths, R. G., Dancer, J., O’ Neill, E., & Harwood, J. L. (2003). A madelamide pesticide alters lipid metabolism in Phytophthora infestans. New Phytologist, 158, 345-353. Grünwald, N. J., Sturbaum, A. K., Romero, G., Garay, E., Lozoya, H., & Fry, W. E. (2006). Selection for Fungicide Resistence within a Growing Season in Field Populations of Phytophthora infestans at the center of origin. Phytopathology, 96(12), 1397-1403. doi: 10.1094/PHYTO-96-1397 Grünwald, N. J., Flier, W. G., Sturbaum, A. K., Garay, E., Van Den Bosch, T. B., Smart, C. D., Matuszak, J. M., Lozoya, H., Turkensteen, L. J. & Fry, W. E. (2001). Population structure of Phytophthora infestans in the Toluca valley region of central Mexico. Phytopathology, 91(9), 882-890. doi: 10.1094/PHYTO.2001.91.9.882 Grünwald, N. J., & Flier, W. G. (2005). The Biology of Phytophthora infestans at Its Center of Origin. Annual Review of Phytopathology, 43, 171-190. doi: 10.1146/annurev.phyto.43.040204.135906 Henfling, J. W. (1987). Late blight of potato: Phytophthora infestans. Technical Information Bulletin 4., Lima Peru: International Potato Center. Judelson, H. S. (1996). Chromosomal heteromorphism linked to the mating type locus of the oomycete Phytophthora infestans. Molecular General Genetics, 252(1-2), 155-161. Kato, M., Mizubuti, E. S., Goodwin, S. B., & Fry, W. E. (1997). Sensitivity to protectant fungicides and pathogenic fitness of clonal lineages of Phytophthora infestans in the United States. Phytopathology, 87(9), 973-980. doi: 10.1094/PHYTO.1997.87.9.973. Knapova, G., Tenzer, I., Gessler, C., & Gisi, U. (2001). Characterization of Phytophthora infestans from potato and tomato with molecular markers. Proceedings of the 5th Congress of the European Foundation for Plant Pathology. Biodiversity in Plant Pathology Taormina, Italy . Locher, F. J., & Lorenz, G. (1991). Methods for monitoring the sensitivity of Botrytis cinerea to ditiocarboximide fungicides. EPPO Bulletin, 21(2), 341-345. doi: 10.1111/j.1365-2338.1991.tb01261.x López-Orona, C. A., Martínez, A. R., Arteaga, T. T., García, H. G., Palmero, D., Ruiz, A., & Peñuelas, C. G. (2013). First report of homothallic isolates of Phytophthora infestans in commercial potato crops (Solanum tuberosum) in the Toluca Valley, Mexico. Plant Disease, 97(8), 1112. doi: 10.1094/PDIS-10-12-0962-PDN Marquinez, R. (1995). Resistance of Phytophthora infestans strains to phenylamides in Spain. In: Dowley, L. J., Bannon, E., Cooke, L. R., Keane, T., & O’Sullivan, E. (Eds.), Phytophthora infestans 150 (pp 137-141). Dublin. Ireland: EAPR, Boole Press Ltd. Matuszak, J. M., Fernández, J., Gu, W. K., Villarreal, M. J., & Fry, W. E. (1994). Sensivity of Phytophthora infestans population to Metalaxyl in México; distribution and dynamics. Plant Disease, 78(9), 911-916. doi: 10.1094/PD-78-0911 Power, R. J., Hamlen, R. A., & Morehart, L. A. (1995). Variation in sensitivity of Phytophthora infestans field isolates to cimoxanil, chlorothalonil and metalaxyl. In: Dowley, L. J., Bannon, E., Cooke, L. R., Keane, T., & O’Sullivan, E. (Eds.), Phytophthora infestans 150 (pp 154-159). Dublin. Ireland: EAPR, Boole Press Ltd . Rekanović, E., Potočnik, I., Milijašević-Marčić, S., Stepanović, M., Todorović, B., & Mihajlović, M. (2011). Toxicity of metalaxyl, azoxystrobin, dimethomorph, cymoxanil, zoxamide and mancozeb to Phytophthora infestans isolates from Serbia. Journal of Environmental Science, 47(5), 403-409. doi: 10.1080/03601234.2012.657043 Robledo-Esqueda, M. N., Lozoya-Saldaña, H., & Colinas-León, M. T. (2012). Inducción de defensa en papa (Solanum tuberosum L.) contra Phytophthora infestans Mont. de Bary, por fungicidas. Interciencia, 37(9), 689-695. Retrieved from http://www.redalyc.org/html/339/33925502006/ Runno, E., & Koppel, M. (2006). The question of metalaxyl resistance on late blight fungus in Estonia. Agronomy Research, 4, 341-344. Retrieved from http://agronomy.emu.ee/vol04Spec/p4S44.pdf Serrano-Cervantes, R.,Lozoya-Saldaña, H.,Colinas y León,M. T. B., & Leyva-Mir, S. G. (2016). Algunas alteraciones enzimáticas en papa causadas por fungicidas. Revista Fitotecnia Mexicana, 39(1), 25-31. Retrieved from http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0187-73802016000100006 Shakya, S. K., Larsen, M. M., Cuenca-Condoy, M. M., Lozoya-Saldaña, H., & Grünwald, N. J. (2016). Microsatellite genotyping reveals high genetic diversity in Phytophthora infestans from Mexico. Proceedings, American Phytopathological Society Annual Meeting, Tampa, Florida, USA. Shaner, G., & Finney, R. E. (1977). The effect of nitrogen fertilization on the expression of slow mildewing resistance in Knox Wheat. Phytopathology, 67, 1051-1056. doi: 10.1094/Phyto-67-1051 Shattock, R. C. (1988). Studies on the inheritance of resistance to metalaxyl in Phytophthora infestans. Plant Pathology, 37(1), 4-11. doi: 10.1111/j.1365-3059.1988.tb02188.x Sozzi, D., Schwinn, F. J., & Gisi, U. (1992). Determination of the sensitivity of Phytophthora infestans to phenylamides: a leaf disease method. EPPO Bulletin, 22 (2), 306-309. doi: 10.1111/j.1365-2338.1992.tb00499.x Statistical Analysis System (SAS Institute). (2004). SAS/STAT.® 9.1 Cary, NC, USA: Author. Temperli, E., Roos, U. P., & Itohl, H. R. (1990). Actin and tubulin cytoskeletons in germlings of the oomycete fungus Phytophthora infestans. European Journal of Cell Biology, 53(1), 75-88. Ziogas, B. N., Markoglow, A. N., Theodosiou, D. I., Anagnostou, A., & Boutopoulou, S. (2006). A high multi-drug resistance to chemically unrelated oomycete fungicides in Phytophthora infestans. European Journal of Plant Pathology, 115(3), 283-292. doi: 10.1007/s10658-006-9007-6 |
|---|